Phoretix 1D - Automatic SDS PAGE and Western Blot Analysis Software

Advanced 1D gel and Western blot image analysis software

Phoretix 1D is the latest evolution of our 1D software and has been developed in line with industry needs and feedback to deliver the highest-quality data in the shortest amount of time.

We have distilled our twenty years’ worth of experience supporting the life science industry into Phoretix 1D to deliver our most advanced set of analysis tools yet to analyse images derived from Gel Doc devices or densitometers.

Phoretix 1D is the complete solution for analysing gel electrophoresis and Immunoblot images, it offers:

    • Speed. Automate your entire analysis and get results in less than a minute per image.
    • Objectivity. Unmatched automation reduces the need for manual intervention and minimises inter-operator variability.
    • Reproducibility. Consistent analysis results between different users, labs and different imaging devices.
    • Quantitation. Enables reliable, accurate quantitation of gel and blot images.
    • 21 CFR/GMP compliant Meet FDA 21 CFR part 11/EU Annex 11/GAMP5 GMP compliance for compliant labs, from captured image through to final analysis and report production.

Phoretix 1D is compatible with the latest versions of both Windows (currently Windows 11) and macOS operating systems (up to and including Ventura).

Our Phoretix 1D software is trusted by the world’s leading pharmaceutical companies

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Harmonise your lab equipment

Our software is device agnostic, so can help reduce costs as it can be used with your existing laboratory equipment, it also works with all industry-standard image formats:


Phoretix 1D supports 8-bit, 16-bit (recommended) and higher uncompressed TIFF images. Multi-page TIFFs are supported and each page is interpreted as a “channel” (all of which must have the same dimensions and pixel format).


While not recommended because of their low bit-depth and (sometimes) compression artifacts, these image formats can be still be analysed.

Automatic detection and quantification of gel electrophoresis data

Phoretix 1D offers automatic lane and band detection or, for even deeper automation and standardisation of your workflow, user-defined protocols which allow full one-click analysis of gel or Western blot images. This removes the potential for operator error entirely.

Coupled with intuitive background removal using industry-leading algorithms, molecular weight calculation, quantity calibration and normalisation Phoretix 1D has all the modern tools required for comprehensive experimental analysis.

If you’d like a demo to see just how easy it is to use, please get in touch

3D view of electrophoresis gel
automatic band detection mode for Western Blot and electrophoresis gels
Western/Immunoblot background subtraction

Supporting our customers

Here at TotalLab, we are a mixture of PhD-educated life scientists, mathematicians and software developers with decades of experience and we take pride in offering the highest level of support and education resources to all of our customers. Our team of experts are dedicated to ensuring that you have the best experience possible with our software and fully understand the principles behind your images.

Our support team is available to answer any questions you may have and provide prompt and efficient solutions to any issues you encounter. We understand that your time is valuable, which is why we aim to resolve your queries as quickly as possible, ensuring minimal disruption to your workflow.

In addition to our exceptional support, we also offer a range of educational resources to help you make the most of our software. Our extensive library of tutorials and guides will provide you with the knowledge you need to use our software to its fullest potential. Whether you are a seasoned professional or a newcomer to our software, our resources will ensure that you have the skills you need to succeed.

You can access our video tutorials here:

At TotalLab, we know that the event of a software malfunction/inability to use your software can seriously impact your business and cost you time and money. We are committed to providing you with the support and resources you need to succeed. Whether you are looking for technical support, educational resources, or simply a friendly chat, our team is here for you.

Supporting 21 CFR Part 11 compliance in GxP laboratories / environments

Phoretix 1D, along with all TotalLab software, can be integrated into our 21 CFR Part 11 compliant GxP Module for regulated environments where data traceability, accountability, and integrity are critical.

Our GxP Module features access control through secure sign-ins, audit logs, electronic signatures and image authenticity checks. Click here to find out more.

Aftersales support

When you purchase from TotalLab, not only do you get access to our industry-leading advanced image analysis software but you also benefit from unlimited technical support, training and free updates to the latest version of your software on release, all included within your subscription.

If you have a question about using our software, please check our FAQ or Resources. Alternatively, you can email and a member of our team will be in touch to help you.

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