Advanced Band Matching for Reliable Analysis
Formally Phoretix 1D Pro, our CLIQS 1D Pro software is an innovative solution for researchers and scientists who work with electrophoresis gels and immunoblots. With its advanced algorithms and accurate band-matching capabilities, CLIQS 1D Pro helps users quickly and easily identify and quantify proteins of interest, even in complex gel and blot images. The software also includes a range of advanced tools and features, such as automatic lane and band detection, background subtraction, and 1D gel lane normalization.
One of the key features of CLIQS 1D Pro is its powerful databasing capabilities. The software allows users to easily create and manage a comprehensive database of lanes and bands, including their molecular weights and other important characteristics. This makes it easy to track and analyse changes in protein expression over time, across different experimental conditions or identify unknown samples by comparing their profiles against those stored in your database
In addition, CLIQS 1D Pro is designed to be user-friendly and intuitive, with a streamlined workflow that simplifies the analysis process.
Compare lanes from multiple gels and experiments
The CLIQS 1D Pro software is incredibly powerful as it allows a completely flexible approach to matching. Each individual lane can be compared to any other lane within the database. The results of matching across many gels can be presented as a dendrogram or tables that show band and lane similarities.
CLIQS 1D Pro can be used to enhance a variety of research techniques including (but not limited to):
- DNA Fingerprinting
- Pulsed-Field Gel Electrophoresis (PFGE)
- Denaturing Gradient Gel Electrophoresis (DGGE)
- Restriction Fragment Length Polymorphism (RFLP)
- Enterobacterial repetitive intergenic consensus (ERIC)-PCR results
- Single-Strand Conformation Polymorphism (SSCP) Fingerprinting
- Automated Approach for Ribosomal Intergenic Spacer Analysis (ARISA)-PCR
Identify lane relationships across multiple experiments
Our software allows you to easily visualise your matching results using dendrograms, which allow the study of relationships between lanes stored in the database.
Once built, the dendrogram is scalable and fully interactive, to allow a flexible presentation of lane relationships. The dendrogram also allows you to create groups of related lanes that can then be reported on in a cluster table.
Identify unknown samples
Lanes containing unknown samples can be matched against a defined reference library of identified samples. Maintenance of the library is very straightforward, and it can be easily shared with co-workers to facilitate collaboration on large projects. This is especially useful in projects based around food security and verification to ensure that the meat content of foods entering the supply chain is from the species claimed.
Case Study: University of Florida
In this collaborative study, we are studying the role of microbial communities, mainly bacteria and fungi in sustainable agricultural practices. The study also aims to establish how microbial communities can be used as indicators of success in sustainable agriculture settings and plant disease management. To achieve these objectives, bacterial and fungal communities in different types of crop rotations, fertilizer treatments and irrigation regimes were compared.
“By combining ARISA-PCR with this software, we have a versatile tool that can analyze all types of images, allowing the comparison of images from different experiments; it is very quick and easy to use. More importantly it allows me to easily export the data matrix for further statistical analysis.
This means our images are no longer just visual, but, we can extract data that can be used in statistical tests.”
“In our case using ARISA (on an Agilent Bioanalyzer platform) and TotalLab’s CLIQS 1D Pro software has enabled us to perform robust experiments. My advice for any interested user is simple; download the fully functional trial version, try it and you will be impressed. A search of the impressive list of scholarly articles that cite the software will definitely inform you of its ability.”
Case Study: Royal Society for Public Health’s CCFRA Food Research Association
Rapid wheat varietal identification using the Agilent 2100 bioanalyzer and automated pattern-matching
Established in 1856, Royal Society for Public Health (RSPH) is an independent campaigning and educational charity dedicated to improving and protecting the health of the public – both in the United Kingdom and around the world.
“Our study has demonstrated that using the Agilent 2100 bioanalyzer with the CLIQS 1D Pro system offers a standardized, objective method for rapid varietal discrimination. The ease of use and total analysis time of less than 50 minutes makes it most suitable for mill intake use. The optimized system will enable millers to make more confident decisions in accepting grain consignments, and could become widely adopted as an effective policing tool within the grain industry. A number of UK mills have purchased the combined systems for screening wheat deliveries at intake.”
Our CLIQS 1D Pro software has been in active development since the early 2000’s and in that time has been cited numerous times in academic publications.
You can find a comprehensive list of papers published using CLIQS 1D Pro or its predecessor, Phoretix 1D Pro, here.